Coronary Sinus Integrated Lactate Decreases After Ischemia and Reperfusion with GGA Induced hsp70

Purpose: BackgroundIschemia reperfusion(IR) increases lactate.No one has examined if cardiac-specific coronary sinus lactate(CSL)can be reduced with prior cytoprotective heat shock protein 70(hsp70i) induction. We previous demonstrated improved IR in vivo inducted hsp70i.Geranylgeranylacetone(GGA), an hsp70i inducer, never been administered IV preischemically. Interventions to decrease CSL may improve clinical parameters.MethodsRabbit hearts underwent 30 cold cardioplegic ischemia then 60 min reperfusion. One group received IVGGA 24 hours prior(GGA+) and the other vehicle(GGA-). CSlactate was collected throughout reperfusion.We aimed determine effects on myocardial lactate.Hsp70 western blot performed.ResultsBaseline similar between GGA+ GGA-(Figure 1). Both peaked at 1 minute However peak less. At every time point continued reperfusion however GGA- increased later. Integrated CS lactate area less for GGA+(Figure 1).ConclusionIn summary, protective resulted five benefits: peak,decreased faster early reperfusion, all points, does not have a second rise lastly results overall integrated production GGA+. GGA induced hsp70. applications endothelial protection COVID. Ischemia lactate. No lactate(CSL) can hsp70i. Geranylgeranylacetone(GGA), parameters. Rabbit Hsp70 performed. Baseline In